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Deacetoxycephalosporin C synthase (expandase): Research progress and application potential.

ABSTRACT: Cephalosporins play an indispensable role against bacterial infections. Deacetyloxycephalosporin C synthase (DAOCS), also called expandase, is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form the hexavalent thiazide ring of cephalosporin. DAOCS in fungus Acremonium chrysogenum was identified as a bifunctional enzyme with both ring expansion and hydroxylation, whereas two separate enzymes in bacteria catalyze these two reactions. In this review, we briefly summarize its source and function, improvement of the conversion rate of penicillin to deacetyloxycephalosporin C through enzyme modification, crystallography features, the prediction of the active site, and application perspective.

SUBMITTER: Niu X

PROVIDER: S-EPMC8626558 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Deacetoxycephalosporin C synthase (expandase): Research progress and application potential.

Niu Xiaofan X Zhang Jian J Xue Xianli X Wang Depei D Wang Lin L Gao Qiang Q

Synthetic and systems biotechnology 20211123 4

Cephalosporins play an indispensable role against bacterial infections. Deacetyloxycephalosporin C synthase (DAOCS), also called expandase, is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form the hexavalent thiazide ring of cephalosporin. DAOCS in fungus <i>Acremonium chrysogenum</i> was identified as a bifunctional enzyme with both ring expansion and hydroxylation, whereas two separate enzymes in bacteria catalyze these two reactions. In this review, we briefly summar ...[more]

PMID: 34901478

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