Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain.

ABSTRACT:

Background

Little is known about chronic cannabis smoking-associated oral microbiome and its effects on central nervous system (CNS) functions.

Methods

In the current study, we have analyzed the saliva microbiome in individuals who chronically smoked cannabis with cannabis use disorder (n = 16) and in non-smoking controls (n = 27). The saliva microbiome was analyzed using microbial 16S rRNA sequencing. To investigate the function of cannabis use-associated oral microbiome, mice were orally inoculated with live Actinomyces meyeri, Actinomyces odontolyticus, or Neisseria elongata twice per week for six months, which mimicked human conditions.

Findings

We found that cannabis smoking in humans was associated with oral microbial dysbiosis. The most increased oral bacteria

SUBMITTER: Luo Z 

PROVIDER: S-EPMC8626580 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature