Exercise downregulates HIPK2 and HIPK2 inhibition protects against myocardial infarction.

ABSTRACT:

Background

Exercise can protect myocardial infarction (MI) and downregulate cardiac Homeodomain-Interacting Protein Kinase 2 (HIPK2). However, the role of HIPK2 in MI is unclear.

Methods

HIPK2^-/- mice and miR-222^-/- rats, HIPK2 inhibitor (PKI1H) and adeno-associated virus serotype 9 (AAV9) carrying miR-222 were applied in the study. Animals were subjected to running, swimming, acute MI or post-MI remodeling. HIPK2 inhibition and P53 activator were used in neonatal rat cardiomyocytes (NRCMs) and human embryonic stem cell-derived cardiomyocytes (hESC-CMs) subjected to oxygen glucose deprivation/reperfusion (OGD/R). Serum miR-222 levels were analyzed in healthy people and MI patients that were survival or readmitted to the hospital and/or died.

Findings

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC8626841 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature