Metformin attenuates LPS-induced neuronal injury and cognitive impairments by blocking NF-κB pathway.

ABSTRACT:

Background

Neuroinflammatory response is considered to be a high-risk factor for cognitive impairments in the brain. Lipopolysaccharides (LPS) is an endotoxin that induces acute inflammatory responses in injected bodies. However, the molecular mechanisms underlying LPS-associated cognitive impairments still remain unclear.

Methods

Here, primary hippocampal neurons were treated with LPS, and western blotting and immunofluorescence were used to investigate whether LPS induces neurons damage. At the same time, SD rats were injected with LPS (830 μg/Kg) intraperitoneally, and Open field test, Novel Objective Recognition test, Fear condition test were used to detect cognitive function. LTP was used to assess synaptic plasticity, and molecular biology technology was used to assess

SUBMITTER: Zhou C 

PROVIDER: S-EPMC8626880 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature