Baiting out a full length sequence from unmapped RNA-seq data.

ABSTRACT:

Background

As a powerful tool, RNA-Seq has been widely used in various studies. Usually, unmapped RNA-seq reads have been considered as useless and been trashed or ignored.

Results

We develop a strategy to mining the full length sequence by unmapped reads combining with specific reverse transcription primers design and high throughput sequencing. In this study, we salvage 36 unmapped reads from standard RNA-Seq data and randomly select one 149 bp read as a model. Specific reverse transcription primers are designed to amplify its both ends, followed by next generation sequencing. Then we design a statistical model based on power law distribution to estimate its integrality and significance. Further, we validate it by Sanger sequencing. The result shows that the full length is

SUBMITTER: Li D 

PROVIDER: S-EPMC8626966 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature