Metabolic imaging of human cumulus cells reveals associations among metabolic profiles of cumulus cells, patient clinical factors, and oocyte maturity.

ABSTRACT:

Objective

To determine whether fluorescence lifetime imaging microscopy (FLIM) detects differences in metabolic state among cumulus cell samples and whether their metabolic state is associated with patient age, body mass index (BMI), and antimüllerian hormone (AMH) level and maturity of the oocyte.

Design

Prospective observational study.

Setting

Academic laboratory.

Patient(s)

Cumulus cell (CC) clusters from cumulus-oocyte complexes were collected from patients undergoing assisted reproductive technology treatment after oocyte retrieval and vitrified.

Intervention(s)

Cumulus cell metabolism was assessed using FLIM to measure autofluorescence of nicotinamide adenine (phosphate) dinucleotide and flavine adenine dinucleotide, endogenous coenzymes essential

SUBMITTER: Venturas M 

PROVIDER: S-EPMC8627436 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature