A Swine Hind Limb Ischemia Model Useful for Testing Peripheral Artery Disease Therapeutics.

ABSTRACT: Currently, there is no large animal model of sustained limb ischemia suitable for testing novel angiogenic therapeutics for peripheral artery disease (PAD) such as drugs, genes, materials, or cells. We created a large animal model suitable for efficacy assessment of these therapies by testing 3 swine hind limb ischemia (HLI) variations and quantifying vascular perfusion, muscle histology, and limb function. Ligation of the ipsilateral external and bilateral internal iliac arteries produced sustained gait dysfunction compared to isolated external iliac or unilateral external and internal iliac artery ligations. Hyperemia-dependent muscle perfusion deficits, depressed limb blood pressure, arteriogenesis, muscle atrophy, and microscopic myopathy were quantifiable in ischemic limbs 6 weeks pos

SUBMITTER: Deppen JN 

PROVIDER: S-EPMC8627534 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature