Contribution of Glucosylceramide Synthase to the Proliferation of Mouse Osteoblasts.

ABSTRACT:

Background/aim

Glycosphingolipids are known to be involved in bone metabolism. However, their roles and regulatory mechanisms in osteoblast proliferation are largely unknown. In this study, we examined the effects of inhibitors of glucosylceramide synthase (GCS), which is responsible for the generation of all glycosphingolipids, on osteoblast proliferation.

Materials and methods

We analyzed the expression of glycosphingolipids and cell growth in MC3T3-E1 mouse osteoblast cells treated with the GCS inhibitors miglustat, D-PDMP and D-PPMP. We also conducted microarray analysis and RNA interference to identify genes involved in cell growth regulated by GCS.

Results

Glycosphingolipids GD1a and Gb4 expressed in MC3T3-E1 cells, were suppressed by GCS inhibitors. Furthermore

SUBMITTER: Mishima Y 

PROVIDER: S-EPMC8627765 | biostudies-literature | 2021 Nov-Dec

REPOSITORIES: biostudies-literature