High resolution estimates of relative gene abundance with quantitative ratiometric regression PCR (qRR-PCR).

ABSTRACT: Quantification of the relative abundance of genetic traits has broad applications for biomarker discovery, diagnostics, and assessing gene expression in biological research. Relative quantification of genes is traditionally done with the 2^-ΔΔCT method using quantitative real-time polymerase chain reaction (qPCR) data, which is often limited in resolution beyond orders of magnitude difference. The latest techniques for quantification of nucleic acids employ digital PCR or microarrays which involve lengthy sample preparation and complex instrumentation. In this work, we describe a quantitative ratiometric regression PCR (qRR-PCR) method for computing relative abundance of genetic traits in a sample with high resolution from a single duplexed real-time quantitative PCR assay. Inste

SUBMITTER: Trick AY 

PROVIDER: S-EPMC8627783 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature