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Contribution of the Idylla^TM System to Improving the Therapeutic Care of Patients with NSCLC through Early Screening of EGFR Mutations.

ABSTRACT: Epidermal growth factor receptor (EGFR) genotyping, a critical examen for the treatment decisions of patients with non-small cell lung cancer (NSCLC), is commonly assayed by next-generation sequencing (NGS), but this global approach takes time. To determine whether rapid EGFR genotyping tests by the Idylla^TM system guides earlier therapy decisions, EGFR mutations were assayed by both the Idylla^TM system and NGS in 223 patients with NSCLC in a bicentric prospective study. Idylla^TM demonstrated agreement with the NGS method in 187/194 cases (96.4%) and recovered 20 of the 26 (77%) EGFR mutations detected using NGS. Regarding the seven missed EGFR mutations, five were not detected by the Idylla^TM system, one was assayed in a sample with insufficient tumoral cells, and the last was in a sample not validated by the Idylla^TM system (a bone metastasis). Idylla^TM did not detect any false positives. The average time between EGFR genotyping results from Idylla^TM and the NGS method was 9.2 ± 2.2 working days (wd) (12.6 ± 4.0 calendar days (cd)). Subsequently, based on the Idylla^TM method, the timeframe from tumor sampling to the initiation of EGFR-TKI was 7.7 ± 1.2 wd (11.4 ± 3.1 cd), while it was 20.3 ± 6.7 wd (27.2 ± 8.3 cd) with the NGS method (p < 0.001). We thus demonstrated here that the Idylla^TM system contributes to improving the therapeutic care of patients with NSCLC by the early screening of EGFR mutations.

SUBMITTER: Petiteau C

PROVIDER: S-EPMC8628756 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Contribution of the IdyllaTM System to Improving the Therapeutic Care of Patients with NSCLC through Early Screening of EGFR Mutations.

Petiteau Constance C Robinet-Zimmermann Gwladys G Riot Adèle A Dorbeau Marine M Richard Nicolas N Blanc-Fournier Cécile C Bibeau Frédéric F Deshayes Simon S Bergot Emmanuel E Gervais Radj R Levallet Guénaëlle G

Current oncology (Toronto, Ont.) 20211103 6

Epidermal growth factor receptor (EGFR) genotyping, a critical examen for the treatment decisions of patients with non-small cell lung cancer (NSCLC), is commonly assayed by next-generation sequencing (NGS), but this global approach takes time. To determine whether rapid EGFR genotyping tests by the IdyllaTM system guides earlier therapy decisions, EGFR mutations were assayed by both the IdyllaTM system and NGS in 223 patients with NSCLC in a bicentric prospec ...[more]

PMID: 34898548

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Project description:Background and Objectives: Cervical cancer is one of the most common types of frequently found cancers in Thailand. One of the causative agents is the infection of the high-risk human papillomavirus (HPV) type 16 and 18. Traditional medicines are rich sources of bioactive compounds which are a valuable source for the development of novel cancer therapies. In this study, the therapeutic effects of 3 traditional medicines, KerraTM, KSTM, and MinozaTM, were studied on HeLa and CaSki cells. Materials and Methods: The effects of KerraTM, KSTM, and MinozaTM on cancer cells were evaluated through cytotoxicity and cell death assays. The infection assay using HPV-16 pseudovirus was also carried out. Results: All traditional medicines efficiently suppressed cell growths of HeLa and CaSki, with KerraTM being the most potent anticancer agent followed by KSTM and MinozaTM. KerraTM at 158 µg/mL and 261 µg/mL significantly increases the percentage inhibition of the HPV-16 pseudovirus infection in a pre-attachment step in a dose-dependent manner, while KSTM at 261 µg/mL efficiently inhibited viral infection in both pre-attachment and adsorption steps. However, KerraTM, KSTM, and MinozaTM at subtoxic concentrations could not reduce the viral E6 mRNA expressions of HPV-16 and HPV-18. Cell death assay by acridine orange/ethidium bromide showed that KerraTM increased population of dead cells in dose-dependent manner in both CaSki and HeLa. The percentage of secondary necrosis in KerraTM-treated CaSki was higher than that of HeLa cells, while the percentage of late apoptotic cells in HeLa was higher than that of CaSki, indicating that HeLa was more susceptible to KerraTM than CaSki. For KSTM and MinozaTM, these extracts at 250 µg/mL promoted autophagy over cell death. At 500 µg/mL, the percentage of dead cells in KerraTM was higher than that of KSTM and MinozaTM. Conclusions: KerraTM is a potent traditional medicine for promoting cancer cell death. KerraTM is possibly useful in the prevention and treatment of cervical cancer. Further investigation will be carried out to gain a better understanding of the biochemical mechanism and the pharmacological activity underlying this effect.

Dataset Information

Contribution of the Idylla^TM System to Improving the Therapeutic Care of Patients with NSCLC through Early Screening of EGFR Mutations.

Publications

Contribution of the Idylla<sup>TM</sup> System to Improving the Therapeutic Care of Patients with NSCLC through Early Screening of <i>EGFR</i> Mutations.

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