Comparison of Sample Preparation Methods for Shotgun Proteomic Studies in Aquaculture Species.

ABSTRACT: Proteomics has been recently introduced in aquaculture research, and more methodological studies are needed to improve the quality of proteomics studies. Therefore, this work aims to compare three sample preparation methods for shotgun LC-MS/MS proteomics using tissues of two aquaculture species: liver of turbot Scophthalmus maximus and hepatopancreas of Mediterranean mussel Mytilus galloprovincialis. We compared the three most common sample preparation workflows for shotgun analysis: filter-aided sample preparation (FASP), suspension-trapping (S-Trap), and solid-phase-enhanced sample preparations (SP3). FASP showed the highest number of protein identifications for turbot samples, and S-Trap outperformed other methods for mussel samples. Subsequent functional analysis reveale

SUBMITTER: Araujo MJ 

PROVIDER: S-EPMC8628934 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature