Mass Spectrometric Identification of a Novel Factor XIIIa Cross-Linking Site in Fibrinogen.

ABSTRACT: Transglutaminases are a class of enzymes that catalyze the formation of a protein:protein cross-link between a lysine and a glutamine residue. These cross-links play important roles in diverse biological processes. Analysis of cross-linking sites in target proteins is required to elucidate their molecular action on target protein function and the molecular specificity of different transglutaminase isozymes. Mass-spectrometry using settings designed for linear peptide analysis and software designed for the analysis of disulfide bridges and chemical cross-links have previously been employed to identify transglutaminase cross-linking sites in proteins. As no control peptide with which to assess and improve the mass spectrometric analysis of TG cross-linked proteins was available, we developed

SUBMITTER: Semkova ME 

PROVIDER: S-EPMC8628943 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature