CRNDE/ETS1/GPR17 Facilitates the Proliferation, Migration, and Invasion of Glioma.

ABSTRACT:

Background

Numerous lncRNAs were found as regulatory factors for occurrence and progression of various tumors, but there is still less research on the role of lncRNAs in malignant progression of glioma.

Methods

Bioinformatics analysis analyzed differential genes (DEGs) in the TCGA database. MTT, flow cytometry, and Transwell assays were performed to test the proliferation, apoptosis, migration, and invasion of cells. qRT-PCR and western blot were conducted to detect RNA and protein expressions of each gene, respectively. CHIP assay verified the binding relationship between genes. FISH assayed subcellar location of CRNDE, and xenograft in nude mice was performed for in vivo verification.

Results

CRNDE was upregulated in glioma cells, and overexpression of CRNDE

SUBMITTER: Hu Y 

PROVIDER: S-EPMC8629677 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature