Evaluation of accessible regions of Escherichia coli fimH mRNA through computational prediction and experimental investigation.

ABSTRACT:

Background and objectives

This study aimed to investigate the accessible regions of the fimH mRNA using computational prediction and dot-blot hybridization to increase the effectiveness of antisense anti-virulence therapeutics against Uropathogenic Escherichia coli.

Materials and methods

We predicted the secondary structure of the E. coli fimH mRNA using the Sfold and Mfold Web servers and RNA structure 5.5 program. Considering the predicted secondary structure, accessible regions in mRNA of fimH were determined and oligonucleotides complementary to these regions were synthesized and hybridization activity of those oligonucleotides to the fimH Digoxigenin (DIG) labeled mRNA was assessed with dot-blot hybridization.

Results

When searc

SUBMITTER: Mood EH 

PROVIDER: S-EPMC8629811 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature