Cloning, expression, purification and functional analysis of a specific multi-epitope protein from multi drug resistance Acinetobacter baumannii.

ABSTRACT:

Background and objectives

Immunization is a promising strategy to combat against the life-threatening infections by Multi Drug Resistance Acinetobacter baumannii. In this study, we directed to design and evaluate the efficacy of a recombinant multi-epitope protein against this pathogen.

Materials and methods

Epitopes prediction was performed for candidate proteins OmpA and BAM complex (BamA, BamB, BamC, BamD, BamE) from A. baumannii, using immune-informatics tools with high affinity for the human HLA alleles. After expression and purification of the recombinant protein, its functional activity was confirmed by interaction with positive sera.

Results

Cloning and expression of the desired multi-epitopes protein were verified. Circular Dichroism study showe

SUBMITTER: Davoudi Z 

PROVIDER: S-EPMC8629821 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature