Molecular characterization of Vibrio cholerae O1 strains circulating in Assam: a north eastern state of India.

ABSTRACT:

Background and objectives

Information on the genetic epidemiology of cholera in Assam, a northeastern state of India is lacking despite cholera being a major public health problem. The study aimed to determine the virulence genes and genes encoding antibiotic resistance in Vibrio cholerae isolates and to determine the prevalent genotypes based on the presence or absence of the virulence genes and ctxB genotype.

Materials and methods

Twenty-five V. cholerae strains were subjected to conventional biotyping and serotyping followed by multiplex PCR to detect ctxA, ctxB, zot, ace, O1rfb, tcpA, ompU, ompW, rtxC, hly and toxR and antibiotic resistance genes. Cholera toxin B (ctxB) gene was amplified followed by sequencing.

Results

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SUBMITTER: Sharma A 

PROVIDER: S-EPMC8629826 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature