Dataset Information

Isolation and characterization of bacteriophages from wastewater sources on Enterococcus spp. isolated from clinical samples.

ABSTRACT:

Background and objectives

In recent decades, enterococcal resistance to antimicrobials has greatly increased. Furthermore, these chemicals include several side effects on the patients. Since no reports are available of the bacteriophages' effects on eukaryotic cells, they can be good solutions for multidrug-resistant bacterial problems. Therefore, the major aim of this study was to isolate bacteriophages from wastewaters on clinical antibiotic-resistant enterococci.

Materials and methods

Clinical bacteria were isolated, then enterococcal isolates were identified using different methods. The antibiotic resistance scheme of the enterococcal isolates was assessed. The bacterial isolates were exposed to wastewater samples containing potential bacteriophages. Technically, isolated bacteriophages were studied by electron microscopy.

Results

Isolated bacteria were verified as Enterococcus faecium. Results showed that bacteriophages could easily be isolated from wastewater sources. The isolated bacteriophages were effective on E. faecium as well as Streptococcus dysgalactiae. Furthermore, these bacteriophages were challenged with five other bacteria (ATCC) with no visible effects. In general, the isolated bacteriophages belonged to the Myoviridae, Siphoviridae, and Inoviridae families.

Conclusion

Further studies on bacteriophages and their efficacy on enterococcal strains could increase the treatment possibility of enterococcal infections. Due to these bacteriophages' effects on Streptococcus strains, bacteriophages may be used to treat streptococcal infections as well.

SUBMITTER: Elahi Y

PROVIDER: S-EPMC8629828 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Publications

Isolation and characterization of bacteriophages from wastewater sources on <i>Enterococcus</i> spp. isolated from clinical samples.

Elahi Yara Y Nowroozi Jamileh J Fard Ramin Mazaheri Nezhad RMN

Iranian journal of microbiology 20211001 5

<h4>Background and objectives</h4>In recent decades, enterococcal resistance to antimicrobials has greatly increased. Furthermore, these chemicals include several side effects on the patients. Since no reports are available of the bacteriophages' effects on eukaryotic cells, they can be good solutions for multidrug-resistant bacterial problems. Therefore, the major aim of this study was to isolate bacteriophages from wastewaters on clinical antibiotic-resistant enterococci.<h4>Materials and meth ...[more]

PMID: 34900165

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