Analytical and functional aspects of protein-ligand interactions: Beyond induced fit and conformational selection.

ABSTRACT: Ligand-dependent changes in protein conformation are foundational to biology. Historical mechanistic models for substrate-specific proteins are induced fit (IF) and conformational selection (CS), which invoke a change in protein conformation after ligand binds or before ligand binds, respectively. These mechanisms have important, but rarely discussed, functional relevance because IF vs. CS can differentially affect a protein's substrate specificity or promiscuity, and its regulatory properties. The modern view of proteins as conformational ensembles in both ligand free and bound states, together with the realization that most proteins exhibit some substrate promiscuity, demands a deeper interpretation of the historical models and provides an opportunity to improve mechanistic analyses. Her

SUBMITTER: Redhair M 

PROVIDER: S-EPMC8629920 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature