EIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

ABSTRACT: During autophagy, the coordinated actions of autophagosomes and lysosomes result in the controlled removal of damaged intracellular organelles and superfluous substrates. The evolutionary conservation of this process and its requirement for maintaining cellular homeostasis emphasizes the need to better dissect the pathways governing its molecular regulation. In our previously performed high-content screen, we assessed the effect of 1530 RNA-binding proteins on autophagy. Among the top regulators, we identified the eukaryotic translation initiation factor 4A-3 (eIF4A3). Here we show that depletion of eIF4A3 leads to a potent increase in autophagosome and lysosome biogenesis and an enhanced autophagic flux. This is mediated by the key autophagy transcription factor, TFEB, which becomes depho

SUBMITTER: Sakellariou D 

PROVIDER: S-EPMC8630043 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature