Effects of Simulated Microgravity on Wild Type and Marfan hiPSCs-Derived Embryoid Bodies.

ABSTRACT:

Background

Mechanical unloading in microgravity is thought to induce tissue degeneration by various mechanisms, including the inhibition of regenerative stem cell differentiation. In this work, we investigate the effects of microgravity simulation on early lineage commitment of hiPSCs from healthy and Marfan Syndrome (MFS; OMIM #154700) donors, using the embryoid bodies model of tissue differentiation and evaluating their ultra-structural conformation. MFS model involves an anomalous organization of the extracellular matrix for a deficit of fibrillin-1, an essential protein of connective tissue.

Methods

In vitro models require the use of embryoid bodies derived from hiPSCs. A DRPM was used to simulate microgravity conditions.

Results

Our data suggest an increas

SUBMITTER: Spitalieri P 

PROVIDER: S-EPMC8630351 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature