Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods.

ABSTRACT: The potential biomarkers of Parkinson's disease are α-synuclein and neurofilament light chain (NFL). However, inconsistent preanalytical preparation of plasma could lead to variations in levels of these biomarkers. Different types of potassium salts of EDTA and different centrifugation temperatures during plasma preparation may affect the results of α-synuclein and NFL measurements. In this study, we prepared plasma from eight patients with Parkinson's disease (PD) and seven healthy controls (HCs) by using di- and tri-potassium (K₂- and K₃-) EDTA tubes and recruited a separated cohort with 42 PD patients and 40 HCs for plasma samples prepared from whole blood by centrifugation at room temperature and 4°C, respectively, in K₂-EDTA tubes. The plasma levels of

SUBMITTER: Chang KH 

PROVIDER: S-EPMC8630588 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature