Single-cell expression quantitative trait loci (eQTL) analysis of SLE-risk loci in lupus patient monocytes.

ABSTRACT:

Background

We performed expression quantitative trait locus (eQTL) analysis in single classical (CL) and non-classical (NCL) monocytes from patients with systemic lupus erythematosus (SLE) to quantify the impact of well-established genetic risk alleles on transcription at single-cell resolution.

Methods

Single-cell gene expression was quantified using qPCR in purified monocyte subpopulations (CD14⁺⁺CD16^- CL and CD14^dimCD16⁺ NCL) from SLE patients. Novel analysis methods were used to control for the within-person correlations observed, and eQTLs were compared between cell types and risk alleles.

Results

The SLE-risk alleles demonstrated significantly more eQTLs in NCLs as compared to CLs (p = 0.0004). There were 18 eQTLs excl

SUBMITTER: Ghodke-Puranik Y 

PROVIDER: S-EPMC8630910 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature