Clinical and Molecular Study of the NOG Gene in Families with Mandibular Micrognathism.

ABSTRACT:

Objectives

Previous studies showed that noggin gene (NOG) sequence alterations, as well as epigenetic factors, could influence mandibular development. The aim of this study was to analyze clinical characteristics, NOG gene sequences, and promoter methylation sites in patients with mandibular micrognathism.

Materials and methods

A total of 35 individuals of five Colombian families were subject to clinical and cephalometric analysis for mandibular micrognathism. One nonaffected individual of each family was included as a control. DNA was isolated from whole blood sample from all individuals by salting out method. Nine NOG gene fragments were amplified by polymerase chain reaction (PCR) and sequenced. Identification of CpG islands for methylation analysis a

SUBMITTER: Gutierrez-Prieto SJ 

PROVIDER: S-EPMC8630938 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature