Patterns of pretreatment drug resistance mutations of very early diagnosed and treated infants in Botswana.

ABSTRACT:

Objective

To describe the occurrence of HIV drug resistance mutations (DRMs) in both intact and defective HIV-1 cell-associated DNA (HIV-1 CAD) among early-treated infants.

Design

The Botswana EIT Study (ClinicalTrials.gov NCT02369406) initiated antiretroviral therapy (ART) in the first week of life and evaluated HIV-1 in plasma and peripheral blood mononuclear cells (PBMCs).

Methodology

We analyzed 257 near-HIV-1 full-length sequences (nFLS) obtained by Illumina next-generation sequencing from infants near birth. Sanger sequencing of pol was performed for mothers at delivery and children with clinical failure through 96 weeks. DRMs were identified using the Stanford HIV Drug Resistance Database.

Results

In 27 infants, median PBMC HIV-1 proviral load was 492 co

SUBMITTER: Moraka NO 

PROVIDER: S-EPMC8631156 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature