Nuclear Resonance Vibrational Spectroscopic Definition of the Fe(IV)₂ Intermediate Q in Methane Monooxygenase and Its Reactivity.

ABSTRACT: Methanotrophic bacteria utilize the nonheme diiron enzyme soluble methane monooxygenase (sMMO) to convert methane to methanol in the first step of their metabolic cycle under copper-limiting conditions. The structure of the sMMO Fe(IV)₂ intermediate Q responsible for activating the inert C-H bond of methane (BDE = 104 kcal/mol) remains controversial, with recent studies suggesting both "open" and "closed" core geometries for its active site. In this study, we employ nuclear resonance vibrational spectroscopy (NRVS) to probe the geometric and electronic structure of intermediate Q at cryogenic temperatures. These data demonstrate that Q decays rapidly during the NRVS experiment. Combining data from several years of measurements, we derive the NRVS vibrational features of intermed

SUBMITTER: Jacobs AB 

PROVIDER: S-EPMC8631202 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature