Artificial Selection on Microbiomes To Breed Microbiomes That Confer Salt Tolerance to Plants.

ABSTRACT: We develop a method to artificially select for rhizosphere microbiomes that confer salt tolerance to the model grass Brachypodium distachyon grown under sodium salt stress or aluminum salt stress. In a controlled greenhouse environment, we differentially propagated rhizosphere microbiomes between plants of a nonevolving, highly inbred plant population; therefore, only microbiomes evolved in our experiment, but the plants did not evolve in parallel. To maximize microbiome perpetuation when transplanting microbiomes between plants and, thus, maximize response to microbiome selection, we improved earlier methods by (i) controlling microbiome assembly when inoculating seeds at the beginning of each selection cycle; (ii) fractionating microbiomes before transfer between plants to harvest

SUBMITTER: Mueller UG 

PROVIDER: S-EPMC8631316 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature