Collateral Sensitivity Interactions between Antibiotics Depend on Local Abiotic Conditions.

ABSTRACT: Mutations conferring resistance to one antibiotic can increase (cross-resistance) or decrease (collateral sensitivity) resistance to others. Antibiotic combinations displaying collateral sensitivity could be used in treatments that slow resistance evolution. However, lab-to-clinic translation requires understanding whether collateral effects are robust across different environmental conditions. Here, we isolated and characterized resistant mutants of Escherichia coli using five antibiotics, before measuring collateral effects on resistance to other paired antibiotics. During both isolation and phenotyping, we varied conditions in ways relevant in nature (pH, temperature, and bile). This revealed that local abiotic conditions modified expression of resistance against both the antibiotic use

SUBMITTER: Allen RC 

PROVIDER: S-EPMC8631318 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature