New substrates and determinants for tRNA recognition of RNA methyltransferase DNMT2/TRDMT1.

ABSTRACT: Methylation is a common post-transcriptional modification of tRNAs, particularly in the anticodon loop region. The cytosine 38 (C38) in tRNAs, such as tRNA^Asp-GUC, tRNA^Gly-GCC, tRNA^Val-AAC, and tRNA^Glu-CUC, can be methylated by human DNMT2/TRDMT1 and some homologs found in bacteria, plants, and animals. However, the substrate properties and recognition mechanism of DNMT2/TRDMT1 remain to be explored. Here, taking into consideration common features of the four known substrate tRNAs, we investigated methylation activities of DNMT2/TRDMT1 on the tRNA^Gly-GCC truncation and point mutants, and conformational changes of mutants. The results demonstrated that human DNMT2/TRDMT1 preferred substrate tRNA^Gly-GCC in vitro. L-shaped conf

SUBMITTER: Li H 

PROVIDER: S-EPMC8632113 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature