The RNA structurome in the asexual blood stages of malaria pathogen plasmodium falciparum.

ABSTRACT: Plasmodium falciparum is a deadly human pathogen responsible for the devastating disease called malaria. In this study, we measured the differential accumulation of RNA secondary structures in coding and non-coding transcripts from the asexual developmental cycle in P. falciparum in human red blood cells. Our comprehensive analysis that combined high-throughput nuclease mapping of RNA structures by duplex RNA-seq, SHAPE-directed RNA structure validation, immunoaffinity purification and characterization of antisense RNAs collectively measured differentially base-paired RNA regions throughout the parasite's asexual RBC cycle. Our mapping data not only aligned to a diverse pool of RNAs with known structures but also enabled us to identify new structural RNA regions in the malari

SUBMITTER: Alvarez DR 

PROVIDER: S-EPMC8632117 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature