Structural model of the M7G46 Methyltransferase TrmB in complex with tRNA.

ABSTRACT: TrmB belongs to the class I S-adenosylmethionine (SAM)-dependent methyltransferases (MTases) and introduces a methyl group to guanine at position 7 (m⁷G) in tRNA. In tRNAs m⁷G is most frequently found at position 46 in the variable loop and forms a tertiary base pair with C13 and U22, introducing a positive charge at G46. The TrmB/Trm8 enzyme family is structurally diverse, as TrmB proteins exist in a monomeric, homodimeric, and heterodimeric form. So far, the exact enzymatic mechanism, as well as the tRNA-TrmB crystal structure is not known. Here we present the first crystal structures of B. subtilis TrmB in complex with SAM and SAH. The crystal structures of TrmB apo and in complex with SAM and SAH have been determined by X-ray crystallography to 1.9 Å (apo),

SUBMITTER: Blersch KF 

PROVIDER: S-EPMC8632124 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature