Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX.

ABSTRACT: The architectural complexity and heterogeneity of the tumor microenvironment (TME) remains a substantial obstacle in the successful treatment of cancer. Hypoxia, caused by insufficient oxygen supply, and acidosis, resulting from the expulsion of acidic metabolites, are prominent features of the TME. To mitigate the consequences of the hostile TME, cancer cells metabolically rewire themselves and express a series of specific transporters and enzymes instrumental to this adaptation. One of these proteins is carbonic anhydrase (CA)IX, a zinc-containing extracellular membrane bound enzyme that has been shown to play a critical role in the maintenance of a neutral intracellular pH (pH_i), allowing tumor cells to survive and thrive in these harsh conditions. Although CAIX has been cons

SUBMITTER: Lenferink AEG 

PROVIDER: S-EPMC8632296 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature