ATG7 is dispensable for LC3-PE conjugation in thioglycolate-elicited mouse peritoneal macrophages.

ABSTRACT: Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient (^-/-) and atg7^-/-macrophages, we provide evidence that loss of ATG5 but not of ATG7 resulted in LC3-II depletion. Accumulation of LC3-II in elicited atg7^-/- macrophages in response to bafilomycin A₁ validated these data. Furthermore, complete loss of ATG3 in atg7^-/- macrophages demonstrated that ATG7 and ATG3 are dispensable for LC3-PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7^-/-

SUBMITTER: Vujic N 

PROVIDER: S-EPMC8632316 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature