A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE.

ABSTRACT:

Objective

Dysregulation of transfer RNA (tRNA)-derived small noncoding RNA (tsRNA) signatures in human serum has been found in various diseases. Here, we determine whether the signatures of tsRNAs in serum can serve as biomarkers for diagnosis or prognosis of systemic lupus erythematosus (SLE).

Methods

Initially, small RNA sequencing was employed for the screening serum tsRNAs obtained from SLE patients, followed by validation with TaqMan probe-based quantitative reverse transcription-PCR (RT-PCR) assay. Receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic efficacy. The biological functions of tsRNAs were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) assay.

Results

We first analyzed tsRNA signat

SUBMITTER: Yang P 

PROVIDER: S-EPMC8632637 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature