Disease Duration Influences Gene Expression in Neuromelanin-Positive Cells From Parkinson's Disease Patients.

ABSTRACT: Analyses of gene expression in cells affected by neurodegenerative disease can provide important insights into disease mechanisms and relevant stress response pathways. Major symptoms in Parkinson's disease (PD) are caused by the degeneration of midbrain dopamine (mDA) neurons within the substantia nigra. Here we isolated neuromelanin-positive dopamine neurons by laser capture microdissection from post-mortem human substantia nigra samples recovered at both early and advanced stages of PD. Neuromelanin-positive cells were also isolated from individuals with incidental Lewy body disease (ILBD) and from aged-matched controls. Isolated mDA neurons were subjected to genome-wide gene expression analysis by mRNA sequencing. The analysis identified hundreds of dysregulated genes in PD. Res

SUBMITTER: Tiklova K 

PROVIDER: S-EPMC8632647 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature