Enhanced differentiation of functional human T cells in NSGW41 mice with tissue-specific expression of human interleukin-7.

ABSTRACT: Humanized mouse models have become increasingly valuable tools to study human hematopoiesis and infectious diseases. However, human T-cell differentiation remains inefficient. We generated mice expressing human interleukin-7 (IL-7), a critical growth and survival factor for T cells, under the control of murine IL-7 regulatory elements. After transfer of human cord blood-derived hematopoietic stem and progenitor cells, transgenic mice on the NSGW41 background, termed NSGW41hIL7, showed elevated and prolonged human cellularity in the thymus while maintaining physiological ratios of thymocyte subsets. As a consequence, numbers of functional human T cells in the periphery were increased without evidence for pathological lymphoproliferation or aberrant expansion of effector or memory-like T cel

SUBMITTER: Coppin E 

PROVIDER: S-EPMC8632686 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature