Effects of Midazolam on the Development of Adult Leydig Cells From Stem Cells In Vitro.

ABSTRACT:

Background

Midazolam is a neurological drug with diverse functions, including sedation, hypnosis, decreased anxiety, anterograde amnesia, brain-mediated muscle relaxation, and anticonvulsant activity. Since it is frequently used in children and adolescents for extended periods of time, there is a risk that it may affect their pubertal development. Here, we report a potential effect of the drug on the development of Leydig cells (LCs), the testosterone (T)-producing cells in the testis.

Methods

Stem LCs (SLCs), isolated from adult rat testes by a magnetic-activated cell sorting technique, were induced to differentiate into LCs in vitro for 3 weeks. Midazolam (0.1-30 μM) was added to the culture medium, and the effects on LC development were assayed.

Results

Mida

SUBMITTER: Zhao X 

PROVIDER: S-EPMC8632869 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature