Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus.

ABSTRACT: The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in <40 min. The developed assay fulfils the current guidelines recommended by World Health Organisation and is ma

SUBMITTER: Witkowska McConnell W 

PROVIDER: S-EPMC8632961 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature