3D in vitro M2 macrophage model to mimic modulation of tissue repair.

ABSTRACT: Distinct anti-inflammatory macrophage (M2) subtypes, namely M2a and M2c, are reported to modulate the tissue repair process tightly and chronologically by modulating fibroblast differentiation state and functions. To establish a well-defined three-dimensional (3D) cell culture model to mimic the tissue repair process, we utilized THP-1 human monocytic cells and a 3D collagen matrix as a biomimetic tissue model. THP-1 cells were differentiated into macrophages, and activated using IL-4/IL-13 (M_IL-4/IL-13) and IL-10 (M_IL-10). Both activated macrophages were characterized by both their cell surface marker expression and cytokine secretion profile. Our cell characterization suggested that M_IL-4/IL-13 and M_IL-10 demonstrate M2a- and M2c-like subtypes,

SUBMITTER: Sapudom J 

PROVIDER: S-EPMC8633361 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature