The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion.

ABSTRACT: In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and t

SUBMITTER: Haque O 

PROVIDER: S-EPMC8633375 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature