PiggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition.

ABSTRACT: Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

SUBMITTER: Wolff JH 

PROVIDER: S-EPMC8633390 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature