PBP4-mediated β-lactam resistance among clinical strains of Staphylococcus aureus.

ABSTRACT:

Background

PBP4, a low-molecular-weight PBP in Staphylococcus aureus, is not considered to be a classical mediator of β-lactam resistance. Previous studies carried out by our group with laboratory strains of S. aureus demonstrated the ability of PBP4 to produce β-lactam resistance through mutations associated with the pbp4 promoter and/or gene. Recent studies of β-lactam-resistant clinical isolates of S. aureus have reported similar mutations associated with pbp4.

Objectives

To determine if pbp4-associated mutations reported among clinical strains of S. aureus mediate β-lactam resistance.

Methods

The pbp4 promoters and genes bearing mutations from clinical isolates were cloned into a heterologous host. Reporter, growth and Bocillin assays were performed to assess thei

SUBMITTER: Satishkumar N 

PROVIDER: S-EPMC8633438 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature