Capability of Enterococcus faecalis to shield Gram-negative pathogens from aminoglycoside exposure.

ABSTRACT:

Background

Enterococcus faecalis commonly produce aminoglycoside-modifying enzymes (AMEs) and are implicated in polymicrobial infections.

Objectives

To determine if AME-producing E. faecalis is capable of protecting Enterobacteriaceae and Pseudomonas aeruginosa from gentamicin exposure.

Methods

Two Klebsiella pneumoniae isolates, two Escherichia coli isolates, and two Pseudomonas aeruginosa isolates were investigated in monoculture time-kill experiments, and each Gram-negative organism was also evaluated during co-culture with either AME-producing or AME-deficient E. faecalis. A pharmacokinetic/pharmacodynamics analysis that utilized Log Ratio Areas and a Hill-type mathematical model was used to determine if the maximal killing or potency of gentamicin against the Gra

SUBMITTER: McMurtry TA 

PROVIDER: S-EPMC8633450 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature