ChiTaH: a fast and accurate tool for identifying known human chimeric sequences from high-throughput sequencing data.

ABSTRACT: Fusion genes or chimeras typically comprise sequences from two different genes. The chimeric RNAs of such joined sequences often serve as cancer drivers. Identifying such driver fusions in a given cancer or complex disease is important for diagnosis and treatment. The advent of next-generation sequencing technologies, such as DNA-Seq or RNA-Seq, together with the development of suitable computational tools, has made the global identification of chimeras in tumors possible. However, the testing of over 20 computational methods showed these to be limited in terms of chimera prediction sensitivity, specificity, and accurate quantification of junction reads. These shortcomings motivated us to develop the first 'reference-based' approach termed ChiTaH (Chimeric Transcripts

SUBMITTER: Detroja R 

PROVIDER: S-EPMC8633610 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature