Dataset Information

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO₂ Nanotubes Through GCN5 and Wnt/β-Catenin.

ABSTRACT: Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provides the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO₂ nanotubes were subjected to cyclic mechanical stress, and the related mechanisms were explored. The results of alkaline phosphatase staining, real-time PCR, and Western blot showed that cyclic mechanical stress can regulate the expression level of osteogenic differentiation markers in BMSCs on the surface of TiO₂ nanotubes through Wnt/β-catenin. As an important member of the histone acetyltransferase family, GCN5 exerted regulatory effects on receiving mechanical signals. The results of the ChIP assay indicated that GCN5 could activate the Wnt promoter region. Hence, we concluded that the osteogenic differentiation ability of BMSCs on the surface of TiO₂ nanotubes was enhanced under the stimulation of cyclic mechanical stress, and GCN5 mediated this process through Wnt/β-catenin.

SUBMITTER: Liu Y

PROVIDER: S-EPMC8634263 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO<sub>2</sub> Nanotubes Through GCN5 and Wnt/β-Catenin.

Liu Yanchang Y Cheng Wendan W Zhao Yao Y Gao Liang L Chang Yongyun Y Tong Zhicheng Z Li Huiwu H Jing Juehua J

Frontiers in bioengineering and biotechnology 20211115

Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provides the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO<sub>2</sub> nanotubes were subjected to c ...[more]

PMID: 34869255

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Project description:Understanding the electrical conduction properties of a single nanostructure is essential for gaining insight into the fundamental charge transport through 1D materials and also for exploring the collective behavior of an array of such nanostructures. TiO2 nanostructures, such as electrochemically grown nanotubes, have been widely studied in recent times for several applications. The electrolyte plays a vital role in deciding the morphology, which, in turn, governs the charge transport behavior. Here we present a comparative study of the charge transport through a single TiO2 nanotube grown by electrochemical anodization using ethylene glycol and dimethyl sulphoxide electrolytes. The individual nanotubes are assembled into nanodevices using photolithography without relying on complex and sophisticated process like electron beam lithography or focused ion beam deposition. The electric field dependent charge transport properties show Schottky emission at a lower field regime and Poole-Frenkel emission in the higher region. The temperature-dependent electrical conduction (110 K-410 K) is mediated by two thermal activation processes, attributed to shallow impurities in the low-temperature range (T < 230 K) and to the donors at deep intermediate levels at higher temperatures (T > 230 K). The activation energies for EG based nanotubes are found to be higher than those for DMSO nanotubes owing to the double wall morphology of the formed tubes. Also, the study of the electrical breakdown phenomena of these nanotubes reveals three distinct categories of collapse. 'Model A' type breakdown is characterized by a stepwise rise of the current up to the breakdown point and a fall to zero following a non-uniform step by step decrease, which is driven by crack formation near the electrode interface and its propagation. 'Model B' shows a transient rise and fall in current, leading to breakdown due to electromigration, whereas 'Model C' type breakdown observed in a bundle of nanotubes shows a mixed trend of 'Model A' and 'Model B'. The data and analysis provide insight into the current limit through an individual nanotube or bundle of nanotubes and will be useful for designing prototype nanodevices from titania nanostructures.

Dataset Information

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO₂ Nanotubes Through GCN5 and Wnt/β-Catenin.

Publications

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO<sub>2</sub> Nanotubes Through GCN5 and Wnt/β-Catenin.

Similar Datasets

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Dataset Information

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin.

Publications

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO<sub>2</sub> Nanotubes Through GCN5 and Wnt/β-Catenin.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO₂ Nanotubes Through GCN5 and Wnt/β-Catenin.