Ontology highlight
ABSTRACT: Background
The human milk microbiome may contribute to the benefits of breastfeeding by providing bacteria to the infant gastrointestinal tract. Many women pump their milk, but the effect of pumping on the milk microbiome is unknown.Objectives
Our objective was to determine the effects of pumping supplies on the pumped human milk microbiome.Methods
This was an in-home, randomized, crossover trial of 2 collection methods. Women (n = 52) pumped twice within 3.5 h, once with their own breast pumps and milk collection supplies (OWN SUPP) and once with a hospital-grade pump and sterile collection supplies (STER SUPP). Pumping order was randomized. The milk microbiome was characterized by aerobic culturing and 16S ribosomal RNA gene sequencing.Results
Milk collect
SUBMITTER: Reyes SM
PROVIDER: S-EPMC8634608 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature