A Conserved Histophilus somni 23S Intervening Sequence Yields Functional, Fragmented 23S rRNA.

ABSTRACT: Histophilus somni is a Gram-negative bacterial organism that acts as an opportunistic pathogen and is a fastidious member of the Pasteurellaceae family associated with diseases of respiratory, reproductive, cardiac, and other tissues of ruminants. We identified an intervening sequence (IVS) embedded in all five copies of the 23S rRNA gene in the closed genome sequence of the H. somni isolate USDA-ARS-USMARC-63250 that may play an important role in affecting the biology of the organism. Sequencing the RNA from this isolate shows that most of the IVS is cleaved from the transcript, resulting in independent fragments of this structural rRNA that remain functional within the bacterial ribosome. The IVS lies between positions 1170 and 1278 bp of the 3,017-bp gene and exhibits self-comple

SUBMITTER: Harhay GP 

PROVIDER: S-EPMC8635145 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature