Dataset Information

The complete chloroplast genome sequence and phylogenetic analysis of Sudan grass (Sorghum bicolor subsp. drummondii) cultivar Sa (Poaceae) from Anhui province, China.

ABSTRACT: The complete chloroplast genome of Sorghum bicolor subsp. drummondii cultivar Sa (a modern Sudan grass cultivar) was sequenced and analyzed in the present study. The chloroplast genome is 140,754 bp in length and includes a large single-copy region 82,688 bp in length, a small single-copy region 12,503 bp, and two inverted repeat regions 22,782 bp each. The genome contains 104 unique genes, including 4 rRNAs, 29 tRNAs, and 71 protein-coding genes. The phylogenetic analysis showed that Sudan grass cultivar Sa in a clade with five other complete chloroplast genomes of S. bicolor. The work facilitates studies on population genetic structure and phylogenetic relationships in genus Sorghum.

SUBMITTER: Li J

PROVIDER: S-EPMC8635530 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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The complete chloroplast genome sequence and phylogenetic analysis of Sudan grass (<i>Sorghum bicolor subsp. drummondii</i>) cultivar Sa (Poaceae) from Anhui province, China.

Li Jieqin J Wang Lihua L

Mitochondrial DNA. Part B, Resources 20211123 12

The complete chloroplast genome of <i>Sorghum bicolor subsp. drummondii</i> cultivar Sa (a modern Sudan grass cultivar) was sequenced and analyzed in the present study. The chloroplast genome is 140,754 bp in length and includes a large single-copy region 82,688 bp in length, a small single-copy region 12,503 bp, and two inverted repeat regions 22,782 bp each. The genome contains 104 unique genes, including 4 rRNAs, 29 tRNAs, and 71 protein-coding genes. The phylogenetic analysis showed that Sud ...[more]

PMID: 34869873

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Project description:Recent efforts to engineer C4 photosynthetic traits into C3 plants such as rice demand an understanding of the genetic elements that enable C4 plants to outperform C3 plants. As a part of the C4 Rice Consortium's efforts to identify genes needed to support C4 photosynthesis, EMS mutagenized sorghum populations were generated and screened to identify genes that cause a loss of C4 function. Stable carbon isotope ratio (δ13C) of leaf dry matter has been used to distinguishspecies with C3 and C4 photosynthetic pathways. Here, we report the identification of a sorghum (Sorghum bicolor) mutant with a low δ13C characteristic. A mutant (named Mut33) with a pale phenotype and stunted growth was identified from an EMS treated sorghum M2 population. The stable carbon isotope analysis of the mutants showed a decrease of 13C uptake capacity. The noise of random mutation was reduced by crossing the mutant and its wildtype (WT). The back-cross (BC1F1) progenies were like the WT parent in terms of 13C values and plant phenotypes. All the BC1F2 plants with low δ13C died before they produced their 6th leaf. Gas exchange measurements of the low δ13C sorghum mutants showed a higher CO2 compensation point (25.24 μmol CO2.mol-1air) and the maximum rate of photosynthesis was less than 5μmol.m-2.s-1. To identify the genetic determinant of this trait, four DNA pools were isolated; two each from normal and low δ13C BC1F2 mutant plants. These were sequenced using an Illumina platform. Comparison of allele frequency of the single nucleotide polymorphisms (SNPs) between the pools with contrasting phenotype showed that a locus in Chromosome 10 between 57,941,104 and 59,985,708 bps had an allele frequency of 1. There were 211 mutations and 37 genes in the locus, out of which mutations in 9 genes showed non-synonymous changes. This finding is expected to contribute to future research on the identification of the causal factor differentiating C4 from C3 species that can be used in the transformation of C3 to C4 plants.

Dataset Information

The complete chloroplast genome sequence and phylogenetic analysis of Sudan grass (Sorghum bicolor subsp. drummondii) cultivar Sa (Poaceae) from Anhui province, China.

Publications

The complete chloroplast genome sequence and phylogenetic analysis of Sudan grass (<i>Sorghum bicolor subsp. drummondii</i>) cultivar Sa (Poaceae) from Anhui province, China.

Similar Datasets

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