Development and Application of EST-SSR Markers in Cephalotaxus oliveri From Transcriptome Sequences.

ABSTRACT: Cephalotaxus oliveri is an endemic conifer of China, which has medicinal and ornamental value. However, the limited molecular markers and genetic information are insufficient for further genetic studies of this species. In this study, we characterized and developed the EST-SSRs from transcriptome sequences for the first time. The results showed that a total of 5089 SSRs were identified from 36446 unigenes with a density of one SSR per 11.1 kb. The most common type was trinucleotide repeats, excluding mononucleotide repeats, followed by dinucleotide repeats. AAG/CTT and AT/AT exhibited the highest frequency in the trinucleotide and dinucleotide repeats, respectively. Of the identified SSRs, 671, 1125, and 1958 SSRs were located in CDS, 3'UTR, and 5'UTR, respectively. Functional annot

SUBMITTER: Liu H 

PROVIDER: S-EPMC8635753 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature