Live Imaging of Apoptotic Extrusion and Quantification of Apical Extrusion in Epithelial Cells.

ABSTRACT: Apoptotic cell death eliminates unhealthy cells and maintains homeostatic cell numbers within tissues. Epithelia, which serve as fundamental tissue barriers for the body, depend on a physical expulsion of dying cells (apoptotic cell extrusion) to remain sealed and intact. Apoptotic cell extrusion has been widely studied over recent years, with researchers using various approaches to induce apoptotic cell death. Unfortunately, the majority of chemical-based approaches for cell death induction rely on sporadically occurring apoptosis and extrusion, making imagining lengthy, often unsuccessful, and difficult to capture in high-quality images because of the frequent frame sampling needed to visualise the key molecular processes that drive extrusion. Here, we present a protocol that describes s

SUBMITTER: Duszyc K 

PROVIDER: S-EPMC8635854 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature