Microtubules regulate pancreatic β-cell heterogeneity via spatiotemporal control of insulin secretion hot spots.

ABSTRACT: Heterogeneity of glucose-stimulated insulin secretion (GSIS) in pancreatic islets is physiologically important but poorly understood. Here, we utilize mouse islets to determine how microtubules (MTs) affect secretion toward the vascular extracellular matrix at single cell and subcellular levels. Our data indicate that MT stability in the β-cell population is heterogenous, and that GSIS is suppressed in cells with highly stable MTs. Consistently, MT hyper-stabilization prevents, and MT depolymerization promotes the capacity of single β-cell for GSIS. Analysis of spatiotemporal patterns of secretion events shows that MT depolymerization activates otherwise dormant β-cells via initiation of secretion clusters (hot spots). MT depolymerization also enhances secretion from individual cells, intr

SUBMITTER: Trogden KP 

PROVIDER: S-EPMC8635970 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature